²úÆ·ÃèÊö
¿ìËÙÀ©Ôö cDNA Ä©¶Ë£¨RACE£© ÊÇÒ»ÖÖµ± RNA ת¼±¾µÄÐòÁнö²¿·ÖÒÑ֪ʱ£¬ ÒÔ RNA Ϊģ°å¸ßЧÄæתȫ³¤ cDNA£¬ ²¢½« cDNA ×÷Ϊģ°å¿ìËÙÀ©ÔöÆä 5′ Ä©¶Ë»ò 3′ Ä©¶Ë¡£ µÂÌ©ÉúÎï?5¡ä?RACE/3¡ä?RACE PCRÀ©ÔöÊÔ¼ÁºÐ°üº¬5′ RACE À©ÔöºÍ 3′ RACE À©ÔöËùÐè×é·Ö£¬ ¿É¸ù¾ÝʵÑéÐèÇó½øÐÐÑ¡Ôñ¡£
²úÆ·ÓÅÊÆ
- Ô¤»ìÊÔ¼Á£¬²Ù×÷¼ò±ã£¬3?²½Íê³ÉÀ©Ôö£»
- ²úÎï¿ÉÖ±½ÓÓÃÓÚ¿Ë¡¡¢²âÐòÓëÏÂÓι¦ÄÜÑéÖ¤¡£
²úÆ·×é³ÉÓë´¢´æÌõ¼þ
| ×é·ÖÃû³Æ |
Ìå»ý£¨¦ÌL£© |
´¢´æÌõ¼þ |
| dNTPs |
20 |
-20¡æ |
| 5¡ä Primer A |
10 |
-20¡æ |
| 3¡ä Primer A |
10 |
-20¡æ |
| Murine RNase Inhibitor |
4 |
-20¡æ |
| DTT |
10 |
-20¡æ |
| Reverse Transcriptase |
4 |
-20¡æ |
| 2 ¡Á PCR Mix |
250 |
-20¡æ |
| RNase-Free water |
1000 |
-20¡æ |
| 10 ¡Á UPM |
50 |
-20¡æ |
| TS Primer |
10 |
-80¡æ |
| 5 ¡Á RT Buffer |
48 |
-80¡æ |
×Ô±¸²ÄÁÏ
- ×ÜRNA»òÕßmRNAÑù±¾£¨×ÜRNA 10pg-500ng/mRNA 1pg-100ng£©£»
- Õñµ´Æ÷¡¢Ë²Ê±ÀëÐÄ»ú¡¢PCRÒǵÈ;
- 5¡ä GSP Primer or 3¡ä GSP Primer¡£
×¢ÒâÊÂÏî
- PCR²Ù×÷¾ùÔÚ±ùÉϽøÐУ»
- ËùÓÐPCRÊÔ¼Á¼°ºÄ²ÄÐèÎÞøÎÞ¾ú¡£
ÎïÁ÷ƽ̨£ºÄ¬ÈÏΪ¾©¶«ÎïÁ÷¡£ÈôÓÐÆäËûÐèÇó£¬ÇëÁªÏµ¶Ô½ÓÏúÊÛ¡£
·¢»õʱ¼ä£ºÈ·È϶©µ¥²¢Íê³É¸¶¿îºó1~2¸ö¹¤×÷ÈÕÄÚ°²ÅÅ·¢»õ¡£
·¢»õ·½Ê½£º±ù´ü»ò¸É±ù¡£
ËÕ¹«Íø°²±¸32011202001300
ËÕICP±¸2021019379ºÅ-1
|